- 420 pages
- English
- ePUB (mobile friendly)
- Available on iOS & Android
A Laboratory Guide to the Tight Junction
About This Book
A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models.
Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research.
Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases.
- Provides consistent and detailed research methods
- Covers various cell, tissue and animal models
- Includes step-by-step guidance from beginner to sophisticated levels
Frequently asked questions
Information
Introduction
Abstract
Keywords
Cell junction
1.1.1 Junction category
- ā¢ Tight junction (zonula occludens) is a multiprotein complex whose general function is to seal the paracellular space to prevent leakage of solutes and water.
- ā¢ Adherens junction (zonula adherens) is a multiprotein architecture whose general role is cell adhesion.
- ā¢ Desmosome (macula adherens) provides a strong type of cell adhesion and is found in stratified epithelium.
- ā¢ Gap junction (macula communicans) creates an intercellular channel allowing direct molecular exchange from cell to cell.
Cellular localization | Integral protein | Plaque protein | Associated filament | |
---|---|---|---|---|
Tight junction | Epithelial cell Endothelial cell | Claudins Occludin | ZOs | Microfilament (actin) |
Adherens junction | Epithelial cell Endothelial cell Cardiomyocyte Mesenchymal and neural cell | Cadherins Nectins | Catenins | Microfilament (actin) |
Desmosome | Stratified epithelial cell | Desmogleins Desmocollins | Desmoplakin plakoglobin | Intermediate filament (cytokeratin, vimentin, desmin) |
Gap junction | Epithelial cell Endothelial cell | Connexins | ZOs | ā |
1.1.2 Tight junction
1.1.3 Adherens junction
1.1.4 Desmosome
1.1.5 Gap junction
Table of contents
- Cover image
- Title page
- Table of Contents
- Copyright
- Dedication
- Author biography
- Preface
- Acknowledgments
- Chapter 1. Introduction
- Chapter 2. Biochemical approaches for tight junction
- Chapter 3. Biophysical approaches for tight junction
- Chapter 4. Histological approaches for tight junction
- Chapter 5. Light microscopy for tight junction
- Chapter 6. Electron microscopy for tight junction
- Chapter 7. Cell models of tight junction biology
- Chapter 8. Mouse models of tight junction physiology
- Chapter 9. Perspective
- Index