Polymerase Chain Reaction for Biomedical Applications

Ali Samadikuchaksaraei

• Verlag: IntechOpen
• Jahr: 2016
• ISBN:
  9789535141334
• Thema: Biowissenschaften
• Thema: Biochemie

Do you want to know the details that should be taken into consideration in order to have accurate conventional and real-time PCR results? If so, this book is for you. Polymerase Chain Reaction for Biomedical Applications is a collection of chapters for both novice and experienced scientists and technologists aiming to address obtaining an optimized real-time PCR result, simultaneous processing of a large number of samples and assays, performing PCR and RT-PCR on cell lysate without extraction of DNA or RNA, detecting false-positive PCR results, detecting organisms in viral and microbial diseases and hospital environment, following safety assessments of food products, and using PCR for introduction of mutations. This is a must-have book for any PCR laboratory.

Inhaltsverzeichnis

• Polymerase Chain Reaction for Biomedical Applications
• Contents
• Preface
• Chapter 1 Guidelines for Successful Quantitative Gene Expression in Real- Time qPCR Assays
• Chapter 2 High-Throughput Platforms in Real-Time PCR and Applications
• Chapter 3 Hot Cell-Direct PCR Aimed at Specific Cell Detection
• Chapter 4 Regulatory Concern of Polymerase Chain Reaction (PCR) Carryover Contamination
• Chapter 5 Multiplex Polymerase Chain Reaction Assay for Early Diagnosis of Viral Infection
• Chapter 6 The Advantages of Using Multiplex PCR for the Simultaneous Detection of Six Sexually Transmitted Diseases
• Chapter 7 - A Real-Time PCR-Based Diagnostic Test for Organismsin Respiratory Tract Infection
• Chapter 8 - PCR Technique for the Microbial Analysis of Inanimate Hospital Environment
• Chapter 9 - PCR: A Powerful Method in Food Safety Field
• Chapter 10 - Site‐Directed Mutagenesis by Polymerase Chain Reaction