Handbook of HLA Typing Techniques

Kam M. Hui, Jeffrey L. Bidwell

• Editorial: CRC Press
• Año: 2020
• ISBN:
  9781000153392
• Edición: 1
• Categoría: Medicina
• Categoría: Teoria, pratica e riferimenti medici

This book presents an authoritative collection of HLA phenotyping and genotyping techniques to be used at the bench level and as a reference. It includes detailed methodologies, notes on the interpretation of tests, reference material, and appendices.

1

PCR-RFLP

INTRODUCTION

The authors have developed the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method based on the digestion of PCR-amplified DNAs with allele-specific enzymes as a reliable, convenient, and practical HLA class II DNA typing technique,^{1, 2, 3, 4, 5, 6, 7} rather than being based on hybridization with multiple sequence-specific oligonucleotide probes (PCR-SSO) (Figure 1). The modified PCR-RFLP method incorporating informative enzymes, which have a single recognition site in some alleles but none in other alleles in the amplified regions, is simpler and more sensitive because genotypes...