Methods of Enzymatic Analysis V2

Hans-UIrich Bergmeyer

• Éditeur: Academic Press
• Année: 2012
• ISBN:
  9780323161374
• Édition: 2
• Sujet: Biological Sciences
• Sous-sujet: Biochemistry

Methods of Enzymatic Analysis, Volume 2 reviews developments in the determination of enzyme activity, including advances in assay techniques. It discusses the principles on which measurements of enzymes are based, with each chapter including equations and each method consisting of the pipetting protocol. This volume is divided into four parts, each discussing a group of enzymes and their determination. Part I focuses on oxidoreductases, such as sorbitol dehydrogenase, lactate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase, 6-phosphogluconate dehydrogenase, xanthine oxidase, and glutamate dehydrogenase. Part II is concerned with transferases ranging from ornithine carbamoyltransferase and transamidinase to transketolase, transaldolase, UDP-glucuronyltransferase, glutamate-pyruvate transaminase, and phosphotransferases. Part III discusses hydrolases including esterases, glycoside hydrolases, peptidases, and proteinases, whereas Part IV looks at lyases, isomerases, and ligases, such as fructose-1, 6-diphosphate aldolase, 1-phosphofructoaldolase, glucosephosphate isomerase, and tetrahydrofolate formylase. This book is a valuable resource for biochemists as well as students and researchers working in the field of analytical biochemistry.

Table des matières

• Cover image
• Title page
• Table of Contents
• Copyright
• Preface
• From the Preface to the 1st Edition
• Contributors
• Abbreviations
• Abbreviations for Chemical and Biochemical Compounds
• Section C: Methods for Determination of Enzyme Activity
• Index
• Atomic Weights