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Antibody Engineering
Thomas Böldicke
- 312 pagine
- English
- PDF
- Disponibile su iOS e Android
Antibody Engineering
Thomas Böldicke
Informazioni sul libro
Antibody Engineering comprises in vitro selection and modification of human antibodies including humanization of mouse antibodies for therapy, diagnosis, and research. This book comprises an overview about the generation of antibody diversity and essential techniques in antibody engineering: construction of immune, naive and synthetic libraries, all available in vitro display methods, humanization by chain shuffling, affinity maturation techniques, de novo synthesis of antibody genes, colony assays for library screening, construction of scFvs from hybridomas, and purification of monoclonal antibodies by exclusion chromatography. In addition, other topics that are discussed in this book are application and mechanism of single domain antibodies, structural diversity of antibodies, immune-mediated skin reactions induced by TNF-alpha recombinant antibodies, and bioinformatic approaches to select pathogen-derived peptide sequences for antibody targets.
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Indice dei contenuti
- Antibody Engineering
- Contents
- Preface
- Chapter 1 Generation of Antibody Diversity
- Chapter 2 High Affinity Maturated Human Antibodies from Naïve and Synthetic Antibody Repertoires
- Chapter 3 Display Technologies for the Selection of Monoclonal Antibodies for Clinical Use
- Chapter 4 Detailed Protocols for the Selection of Antiviral Human Antibodies from Combinatorial Immune Phage Display Libraries
- Chapter 5 Colony Assay for Antibody Library Screening: Outlook and Comparison to Display Screening
- Chapter 6 Construction and Characteristics of a Recombinant Single- Chain Antibody Fragment against Bacterial Type III Secretion
- Chapter 7 Separation of Monoclonal Antibodies by Analytical Size Exclusion Chromatography
- Chapter 8 The Development of Single Domain Antibodies for Diagnostic and Therapeutic Applications
- Chapter 9 Use, Applications and Mechanisms of Intracellular Actions of Camelid VHHs
- Chapter 10 Structural Diversity Problems and the Solving Method for Antibody Light Chains
- Chapter 11 Immune-Mediated Skin Reactions Induced by Recombinant Antibodies and Other TNF-Alpha Inhibitors
- Chapter 12 Bioinformatics as a Tool to Identify Infectious Disease Pathogen Peptide Sequences as Targets for Antibody Engineering