Part One
Amino Acids as Building Blocks
Chapter 1
Amino Acid Biosynthesis
Emily J. Parker and Andrew J. Pratt
1.1 Introduction
The ribosomal synthesis of proteins utilizes a family of 20 α-amino acids that are universally coded by the translation machinery; in addition, two further α-amino acids, selenocysteine and pyrrolysine, are now believed to be incorporated into proteins via ribosomal synthesis in some organisms. More than 300 other amino acid residues have been identified in proteins, but most are of restricted distribution and produced via post-translational modification of the ubiquitous protein amino acids [1]. The ribosomally encoded α-amino acids described here ultimately derive from α-keto acids by a process corresponding to reductive amination. The most important biosynthetic distinction relates to whether appropriate carbon skeletons are pre-existing in basic metabolism or whether they have to be synthesized de novo and this division underpins the structure of this chapter.
There are a small number of α-keto acids ubiquitously found in core metabolism, notably pyruvate (and a related 3-phosphoglycerate derivative from glycolysis), together with two components of the tricarboxylic acid cycle (TCA), oxaloacetate and α-ketoglutarate (α-KG). These building blocks ultimately provide the carbon skeletons for unbranched α-amino acids of three, four, and five carbons, respectively. α-Amino acids with shorter (glycine) or longer (lysine and pyrrolysine) straight chains are made by alternative pathways depending on the available raw materials. The strategic challenge for the biosynthesis of most straight-chain amino acids centers around two issues: how is the α-amino function introduced into the carbon skeleton and what functional group manipulations are required to generate the diversity of side-chain functionality required for the protein function?
The core family of straight-chain amino acids does not provide all the functionality required for proteins. α-Amino acids with branched side-chains are used for two purposes; the primary need is related to protein structural issues. Proteins fold into well-defined three-dimensional shapes by virtue of their amphipathic nature: a significant fraction of the amino acid side-chains are of low polarity and the hydrophobic effect drives the formation of ordered structures in which these side-chains are buried away from water. In contrast to the straight-chain amino acids, the hydrophobic residues have large nonpolar surface areas by virtue of their branched hydrocarbon side-chains. The other role of branched amino acids is to provide two useful functional groups: an imidazole (histidine) and a phenol (tyrosine) that exploit aromatic functional group chemistry.
This chapter provides an overview of amino acid biosynthesis from a chemical perspective and focuses on recent developments in the field. It highlights a few overarching themes, including the following:
i. The chemical logic of the biosynthetic pathways that underpin amino acid biosynthesis. This chemical foundation is critical because of the evolutionary mechanisms that have shaped these pathways. In particular, the way in which gene duplication and functional divergence (via mutation and selection) can generate new substrate specificity and enzyme activities from existing catalysts [2].
ii. The contemporary use of modern multidisciplinary methodology, including chemistry, enzymology, and genomics, to characterize new biosynthetic pathways.
iii. Potential practical implications of understanding the diverse metabolism of amino acid biosynthesis, especially medicinal and agrichemical applications.
iv. The higher-level molecular architectures that control the fate of metabolites, especially the channeling of metabolites between active sites for efficient utilization of reactive intermediates.
Box 1.1: Nitrogen and Redox in Amino Acid Biosynthesis
Ammonia is toxic and the levels of ammonia available for the biosynthesis of amino acids in most biochemical situations is low. There are a limited number of entry points of ammonia into amino acid biosynthesis, notably related to glutamate and glutamine. Once incorporated into key amino acids, nitrogen is transferred between metabolites either directly or via in situ liberation of ammonia by a multifunctional complex incorporating the target biosynthetic enzyme. The main source of in situ generated ammonia for biosynthesis is the hydrolysis of glutamine by glutaminases. De novo biosynthesis of amino acids, like element fixation pathways in general, is primarily reductive in nature. This may reflect the origins of these pathways in an anaerobic world more than 3 billion years ago.
Box 1.2: The Study of Biosynthetic Enzymes and Pathways
The source of an enzyme for biochemical study has important implications. Most core metabolism has been elaborated by studying a small number of organisms that were chosen for a variety of reasons, including availability, ease of manipulation, ethical concerns, scientific characterization, and so on. These exemplar organisms include the bacterium Escherichia coli, the yeast Saccharomyces cerevisiae, the plant Arabidopsis thaliana, and the rat as a typical mammal. Much of the detailed characterization of amino acid biosynthesis commenced with studies on these organisms. With the rise of genetic engineering techniques, biosynthetic enzymes from a wide variety of sources are available for scientific investigation, and there has been increasing emphasis on working with enzymes and pathways from alternative organisms.
Metabolic diversity is greatest among prokaryotes. One fundamental change in the underlying microbiology that has affected our understanding of pathway diversity has been the appreciation of the deep biochemical distinctions between what are now recognized to be two fundamental domains of prokaryotes: eubacteria and Archaea [3]. The former bacteria include those well known to be associated with disease and fermentation processes; while the latter include many methanogens and extremophiles (prokaryotes that grow in extreme conditions, such as hyperthermophiles that grow at temperatures above 60 °C or halophiles that grow in high ionic strength environments). Bioinformatics approaches are complementing conventional enzymological studies in identifying and characterizing interesting alternative biosynthetic pathways [4]. The greater understanding of microbial and biosynthetic diversity is presenting exciting opportunities for novel discoveries in biosynthesis.
Much of the focus of biosynthetic enzymology now focuses on enzymes from pathogens and hyperthermophiles. The focus on the study of enzymes from pathogens is predicated on the possibility that inhibitors of such enzymes may be useful as pesticides and therapeutic agents. Since humans have access to many amino acids in their food, they have lost the ability to make âdietary essentialâ amino acids that typically require extended dedicated biosynthetic pathways [5]. The biosynthetic enzymes of the corresponding pathways are essential for many pathogens and plants, but not for humans; hence, selective inhibitors of these biosynthetic enzymes are potentially nontoxic to humans, but toxic to undesirable organisms. Enzymes from hyperthermophilic organisms, produced by g...