Protein expression is an increasingly important tool for research on gene function. What is needed is not just a lab manual providing established methods as well as the latest state-of-the-art protocols, but also clear advice on what expression system to choose when.
Expression Systems: Methods Express uniquely fills this need. It covers expression across a broad range of systems, including the following. *Baculovirus expression vectors
*CHO cells
* E. coli
*HEK293-EBNA1 cells
* Lactococcus lactis and other gram positive bacteria
* S. cerevisiae
*transfected insect cells* Pichia pastoris
*mammalian cells using BacMam viruses
*lentiviral vectors
*wheat germ cell-free systemThe book takes the reader through how to make an informed choice of appropriate system, taking into account the protein target, the time involved, the ultimate use of the expressed protein, and the laboratory equipment required. It also provides step-by-step methods for each system. In addition, the book describes the optimisation of expression strategies, expression engineering using ribosome display, and how to select protein variants with improved expression. Every chapter discusses the merits and limitations of the approaches available, describes the key techniques in full practical detail, and provides sensible advice for immediate use at the bench. In summary, Expression Systems: Methods Express is a comprehensive laboratory manual and information resource for researchers at all levels, from postgraduate student to principal investigator.

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Yes, you can access Expression Systems by Michael Dyson, Yves Durocher, Michael Dyson, Yves Durocher in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Genetics & Genomics. We have over one million books available in our catalogue for you to explore.

Information

Publisher

Scion Publishing

Year

2007

ISBN

9781907904400

Topic

Biological Sciences

Subtopic

Genetics & Genomics

Index

Biological Sciences

Contents
Color Section
CHAPTER 1: Expression strategy
CHAPTER 2: Protein expression in Escherichia coli
CHAPTER 3: Expression engineering of synthetic
CHAPTER 4: Refolding proteins from inclusion
CHAPTER 5: Selection of protein variants with improved expression using green fluorescent protein-derived folding and solubility reporters
CHAPTER 6: Protein expression in the wheat-germ cell-free system
CHAPTER 7: Saccharomyces cerevisiae...
CHAPTER 8: Expression of proteins in Pichia pastoris
CHAPTER 9: Improved baculovirus expression vectors
CHAPTER 10: Transient transfection of insect cells for rapid expression screening and protein production
CHAPTER 11: Generation of stable CHO cell lines for protein expression
CHAPTER 12: Transient expression in HEK293-EBNA1 cells
CHAPTER 13: Nisin- and subtilin-controlled gene expression systems for Gram-positive bacteria
CHAPTER 14: Protein production using lentiviral vectors
CHAPTER 15: Expression in mammalian cells using BacMam viruses
APPENDIX 1: List of suppliers
Index