Chemical Reagents for Protein Modification

2nd Edition

Roger L. Lundblad

• Publisher: CRC Press
• Year: 2020
• ISBN:
  9781000697797
• Edition: 1
• Topic: Medicine
• Subtopic: Medical Theory, Practice & Reference

First published in 1991, Chemical Reagents for Protein Modification, 2nd Edition provides a unique combination of theoretical and practical considerations for the use of chemical reagents for site-specific modification of proteins. The book is divided into three sections, with the first section describing general techniques, including information on the organic chemistry of the various modification reactions; the separation and characterization of site-specific modified proteins, including applications to proteins separated by electrophoresis followed by blotting; the specific chemical cleavage of peptide bonds in proteins; the separation of peptides by high-performance liquid chromatography and electrophoresis; and the use of chemical reagents to assess conformational change in proteins. The second section provides an encyclopedic description of reagents and reactions for the site-specific modification of individual amino acid residues in proteins. The final section presents descriptions of the use of chemical reagents to label biologically significant sites in proteins, including enzyme active sites and the use of covalent cross-linking to measure protein-protein interactions. Particular emphasis is placed on the use of photoaffinity reagents. The book will be an extremely useful research tool for all investigators interested in the solution chemistry of proteins.

Table of contents

• Cover
• Title Page
• Copyright Page
• Preface to Second Edition
• The Author
• Table of Contents
• Chapter 1 Site-Specific Chemical Modification of Proteins
• Chapter 2 Amino Acid Analysis
• Chapter 3 Peptide Separation by Reverse-Phase High-Performance Liquid Chromatography
• Chapter 4 Methods for Sequence Determination
• Chapter 5 Chemical Cleavage of Peptide Bonds
• Chapter 6 The Modification of Cysteine
• Chapter 7 The Modification of Cysteine — Cleavage of Disulfide Bonds
• Chapter 8 The Modification of Methionine
• Chapter 9 The Modification of Histidine Residues
• Chapter 10 The Modification of Lysine
• Chapter 11 The Modification of Arginine
• Chapter 12 Chemical Modification of Tryptophan
• Chapter 13 The Modification of Tyrosine
• Chapter 14 The Modification of Carboxyl Groups
• Chapter 15 The Chemical Cross-Linking of Peptide Chains
• Chapter 16 Affinity Labeling
• Index