Covering state-of-the-art technologies and a broad range of practical applications, the Third Edition of Gene Biotechnology presents tools that researchers and students need to understand and apply today's biotechnology techniques. Many of the currently available books in molecular biology contain only protocol recipes, failing to explain the princ

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Yes, you can access Gene Biotechnology by William Wu,Helen H. Zhang,Michael J. Welsh,Peter B. Kaufman in PDF and/or ePUB format, as well as other popular books in Medicine & Biotechnology in Medicine. We have over one million books available in our catalogue for you to explore.

Information

Publisher

CRC Press

Year

2016

ISBN

9781439848326

Edition

Topic

Medicine

Subtopic

Biotechnology in Medicine

Front Cover
Contents
Preface
Authors
Chapter 1: Strategies for Novel Research Projects and/or Research Grant Funding
Chapter 2: Rapid Isolation of Specific cDNAs or Genes by PCR
Chapter 3: Construction and Screening of Subtracted and Complete Expression cDNA Libraries
Chapter 4: Subcloning of Genes or DNA Fragments
Chapter 5: Nonisotopic and Isotopic DNA or RNA Sequencing
Chapter 6: Bioinformation Superhighway and Computer Databases of Nucleic Acids and Proteins
Chapter 7: Characterization of DNA or Genes by Southern Blot Hybridization
Chapter 8: Gene Overexpression by Sense RNA in Mammalian Systems
Chapter 9: Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies
Chapter 10: Analysis of Gene Expression at Functional Genomic Level Using Northern Blotting or PCR
Chapter 11: Analysis of Gene Expression at Proteomic Level via Western Blotting
Chapter 12: Analysis of Cellular DNA or Abundance of mRNA by Radioactivity In Situ Hybridization
Chapter 13: Localization of DNA or Abundance of mRNA by Fluorescence In Situ Hybridization
Chapter 14: In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAs
Chapter 15: Isolation and Characterization of Genes from Genomic DNA Libraries
Chapter 16: Mouse Stem Cells as a Model Mammalian Cell Line for Gene Expression
Chapter 17: Strategies for Gene Double Knockout
Chapter 18: Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells
Chapter 19: Quantitative Analysis of Functional Genome by Real-Time RT-PCR
Chapter 20: High-Throughput Analysis of Gene Expression by Cutting-Edge Technology—DNA Microarrays (Gene Chips)
Chapter 21: Construction and Screening of Human Antibody Libraries Using Phage Display Technology
Chapter 22: Down-Regulation of Gene Expression in Mammalian Systems via siRNA Technology
Chapter 23: Strategies for Gene Cloning, Expression, and Identification of Protein–Protein Interaction
Chapter 24: Conditional Gene Knockout
Chapter 25: How to Write a Research Manuscript for Publication in an English Journal
Chapter 26: How to Protect Your Discovery and Invention : Patent 101
Chapter 27: Determination of Transgene Copy Numbers and Practical Biocalculation
Back Cover