Gene Transfer to Animal Cells

Richard Twyman

• Publisher: Taylor & Francis
• Year: 2004
• ISBN:
  9781135322786
• Edition: 1
• Topic: Biological Sciences
• Subtopic: Biotechnology
• Index: Biological Sciences

Gene transfer to animal cells was first achieved more thanthirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles.

Gene Transfer to Animals Cells addresses this problem by describing the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. A final chapter provides an overview of all the applications of animal cell transformation in farming, medicine and research.

Table of contents

• Cover Page
• Half-title Page
• Title Page
• Copyright Page
• Table of Contents
• Abbreviations
• Preface
• 1 Basic Principles of Gene Transfer
• 2 Chemical and Physical Transfection
• 3 Cell-mediated Gene Transfer
• 4 Gene Transfer Using Viral Vectors
• 5 Genetic Manipulation of Animals
• 6 Gene Targeting in Animal Cells
• 7 Control of Transgene Expression
• 8 Transgene Behavior
• 9 Additional Srategies for Gene Inactivation
• 10 Applications of Gene Transfer in Animals
• Index