Advanced Chromatographic and Electromigration Methods in BioSciences

I. Mikšík, F. Tagliaro, E. Tesarová, Z. Deyl

• Publisher: Elsevier Science
• Year: 1998
• Print ISBN:
  9780444825940
• eBook ISBN:
  9780080858685
• Topic: Physical Sciences
• Subtopic: Analytic Chemistry

This book deals with chromatographic and electrophoretic methods applied for the separation (quantitation and identification) of biologically relevant compounds. It is assumed that the potential reader is familiar with the basics of chromatographic and electromigration methods. Individual separation modes are dealt with to an extent which follows their applicability for biomedical purposes: liquid chromatography and electromigration methods are therefore highlighted.Each chapter is completed with a list of recent literature covering the 1987-1997 period, which can be used for further guidance of the reader in his/her own field. The chapters have been written by specialists in a particular area and with an emphasis on applications to the biomedical field. This implies that theoretical and instrumental aspects are kept to a minimum which allows the reader to understand the text. Considerable attention is paid to method selection, detection and derivatization procedures and troubleshooting. The majority of examples given represent the analyses of typical naturally-occurring mixtures. Adequate attention is paid to the role of the biological matrix and sample pretreatment, and special attention is given to forensic, toxicological and clinical applications. The book is completed with an extensive Index of Compounds Separated.

Table of contents

• Cover image
• Title page
• Table of Contents
• Copyright page
• Preface
• List of Contributors
• Chapter 1: Selection of Chromatographic Methods for Biological Materials
• Chapter 2: Strategies for Electromigration Separations of Biologically Relevant Compounds
• Chapter 3: Sample Preparation
• Chapter 4: Selected Derivatization Reactions
• Chapter 5: Enantioselective Separations
• Chapter 6: Carbohydrates, Polymeric Sugars and their Constituents
• Chapter 7: Carboxylic Acids
• Chapter 8: Herbal Organic Acids
• Chapter 9: Analysis of Lipids by Thin-Layer Chromatography
• Chapter 10: Biogenic Amines
• Chapter 11: Amino Acids
• Chapter 12: Peptides
• Chapter 13: Protein Analysis
• Chapter 14: Nucleic Acids and Their Constituents
• Chapter 15: Compounds Containing Heterocyclic Nitrogen, Oxygen and Sulfur
• Chapter 16: Vitamins
• Chapter 17: Antibiotics
• Chapter 18: Pesticides in Environmental Samples
• Chapter 19: Monitoring of Process Impurities in Drugs
• Chapter 20: Procedures in Hospital Laboratories
• Chapter 21: Toxicological and Forensic Applications
• Chapter 22: Inorganic Analysis and Speciation
• Chapter 23: Common Pitfalls in CE and HPLC
• Index of Compounds Separated
• Jcournal of Chromatography Library