Neuropeptide Technology

Synthesis, Assay, Purification, and Processing

P. Michael Conn

• Publisher: Academic Press
• Year: 2013
• ISBN:
  9781483259499
• Topic: Biological Sciences
• Subtopic: Biology
• Index: Biological Sciences

Methods in Neurosciences, Volume 6: Neuropeptide Technology: Synthesis, Assay, Purification, and Processing describes procedures and tools of assay useful for the identification, purification, and quantification of neuropeptides and their receptors. This volume is divided into four sections— chemical synthesis and biosynthesis; measurement of neuropeptides; purification and characterization; and neuropeptide degrading and processing enzymes. In these sections, this book specifically discusses the synthesis of peptide substrates for protein kinase C; synthesis of glycosyl neuropeptides; and ultrastructural localization of peptides. The measurement of neurokinin B by radioimmunoassay; purification and characterization of neuroendocrine peptides from rat brain; and preparation of glia maturation factor ß are also elaborated. This text likewise covers the assays for arginine/lysine carboxypeptidases and enzymes that metabolize atrial natriuretic peptide. This publication is beneficial to neuroscientists and students researching on the synthesis, assay, purification, and processing of neuropeptides.

Table of contents

• Cover image
• Title page
• Table of Contents
• Copyright page
• Contributors to Volume 6
• Preface
• Methods in Neurosciences
• Chapter [1]: Large-Scale Synthesis of Gonadotropin-Releasing Hormone Antagonists for Clinical Investigations
• Chapter [2]: Synthesis of Peptide Substrates for Protein Kinase C
• Chapter [3]: Synthesis of Glycosyl Neuropeptides
• Chapter [4]: Assays for Biosynthesis of Thyrotropin-Releasing Hormone
• Chapter [5]: Microsequence Analysis of Proteins Purified by Gel Electrophoresis
• Chapter [6]: Preparation of Site-Specific Peptide Immunogens Using Multiple Antigen Peptide Approach System
• Chapter [7]: Subcellular Distribution of Neuropeptides and Measurement of Their in Vitro Release
• Chapter [8]: Analysis of Tachykinin Peptide Family Gene Expression Patterns by Combined High-Performance Liquid Chromatography–Radioimmunoassay
• Chapter [9]: Ultrastructural Localization of Peptides: Comparison of Methods
• Chapter [10]: Measurement of Immune System in Response to Peptides in Central Nervous System
• Chapter [11]: Identification of Functionally Significant Phosphorylation Sites on Neuronal Proteins and Preparation of Antibodies That Recognize Them
• Chapter [12]: Measurement of Brain Peptides: Angiotensin and Atrial Natriuretic Peptide in Tissue and Cell Culture
• Chapter [13]: Regionally Specific Antisera to Human β-Preprotachykinin
• Chapter [14]: Measurement of Neurokinin B by Radioimmunoassay
• Chapter [15]: Radioimmunoassay of Tachykinins
• Chapter [16]: Radioimmunoassay of α-Melanocyte-Stimulating Hormone
• Chapter [17]: Radioimmunoassay of Atrial Natriuretic Peptide
• Chapter [18]: Radioimmunoassay of Cholecystokinin
• Chapter [19]: Secretion and Radioimmunoassay of Somatostatin: In Vitro System
• Chapter [20]: Purification and Characterization of Immunoregulatory Peptides from Neuroendocrine Tissues: Suppressin as a Model
• Chapter [21]: Purification and Characterization of Neuroendocrine Peptides from Rat Brain: Prosomatostatin Isolation
• Chapter [22]: Preparation of Glia Maturation Factor β
• Chapter [23]: Detection and Purification of Thyrotropin-Releasing Hormone Precursor Peptides Using Antisera Generated against Synthetic Peptides
• Chapter [24]: Physiologic Roles of Peptides as Central Nervous System Transmitters
• Chapter [25]: Assays for Arginine/Lysine Carboxypeptidases: Carboxypeptidases H (E; Enkephalin Convertase), M, and N
• Chapter [26]: Assay of Peptidase That Removes Carboxy-Terminal Tripeptide from 125I-Labeled Atrial Natriuretic Factor
• Chapter [27]: Assays for Enzymes That Metabolize Atrial Natriuretic Peptide
• Index