Cell Culture

P. Michael Conn, P. Michael Conn

• Publisher: Academic Press
• Year: 2013
• ISBN:
  9781483262185
• Topic: Biological Sciences
• Subtopic: Science Research & Methodology
• Index: Biological Sciences

Methods in Neurosciences, Volume 3: Quantitative and Qualitative Microscopy is a collection of papers that deals with microscopic techniques in statistical measures. This volume describes microscopy using sophisticated stains and dyes to advance observation of tests and experiments. Section I describes autoradiography including micro chemical methods, high-resolution autoradiography, and single- or double-label quantitative autoradiography for use in imaging of brain activity patterns or determining cerebral physiology. Section II discusses the quantification of structures through statistical and computational methods including dynamic video imaging technology. Section III explains the use of tracers, toxins, or dyes in tracing neuronal connections. One paper addresses the use of small injections of axonally transported fluorescent tracers. Section IV explains staining technology such as using the silver impregnation method for frozen sections of human nervous tissue that are gathered from tissues preserved in formalin. Section V addresses freezing techniques and those using freeze-fracture methods in neurobiology. The text also discusses cryoprotection and other freezing methods to control ice crystals found in fixed or unfixed brain tissues. Section VI presents the combined and high-resolution methods in polarization microscopy and microscopic investigations. Cellular biologists, micro-chemists, and scientific researchers in the field of micro- and cellular biology will appreciate this book.

Index

A

Acetylcholine

metabolism by primary cultured neurons during in vitro growth, 105–208

receptor levels in microcarrier-based cultures of nerve cells, myoblasts, and autologous nerve–muscle cells, 206

Adenohypophyseal tissue

in vivo culture

applications, 322–328

infusion/withdrawal system, 317–320

maintenance of catheter patency, 321

number of, 321

transplantation into hypophysectomized hosts

procedure, 317

surgical precautions, 321

survival of transplant, 320–321

verification of hypophysectomy, 322

Affinity methods, purification of neurons from chick sympathetic ganglia, 155

Agar medium, semisolid, culture of glioma cells in, 227

Aggregates

fetal rat brain cells

in stationary culture, 219–220

as targets in confrontation cultures, 225–226

formation in neuronal and muscle microcarrier cultures, 202–203

D-Ala²-D-Leu⁵-enkephalin, chronic exposure of cord-DRG expiants to, 80

Amino acid sequences

bombesin, 342

epidermal growth factor (human), 342

gastrin-releasing peptide (human), 342

γ-Aminobutyric acid

differential sensitivity of sensory-evoked synaptic network responses of cord-DRG explants, 78

effect on [³H]flunitrazepam binding to primary cultured neurons, 108 metabolism by primary cultured neurons during

in vitro growth, 105–208

Amino-dextran carriers...

Table of contents

• Cover image
• Title page
• Table of Contents
• Copyright page
• Contributors to Volume 2
• Preface
• Methods in Neurosciences
• Section I: General Approaches to Preparation of Cell Cultures
• Section II: Purification of Cell Types
• Section III: Bulk Isolation
• Section IV: Special Approaches
• Section V: Functional Assessment
• Section VI: Cell Lineage
• Index