Gene Transfer and Expression in Mammalian Cells

S.C. Makrides

• Publisher: Elsevier Science
• Year: 2003
• ISBN:
  9780080930350
• Topic: Biological Sciences
• Subtopic: Molecular Biology
• Index: Biological Sciences

The production of proteins in mammalian cells is an important tool in numerous scientific and commercial areas. For example, proteins for human therapy, vaccination or diagnostic applications are typically produced in mammalian cells. Gene cloning, protein engineering, biochemical and biophysical characterization of proteins also require the use of gene expression in mammalian cells. Other applications in widespread use involve screening of libraries of chemical compounds in drug discovery, and the development of cell-based biosensors.This book presents a state-of-the-art comprehensive coverage of the technical aspects of gene expression in mammalian cells, written by experienced scientists working at the forefront of the field.

Subject Index

Accessory genes 29

N-Acetylglucosamine (GlcNAc) 434, 435

O-β-N-Acetylglucosaminyltransferases (OGT) 444

Acquired immunodeficiency syndrome (AIDS) 88, 169

criteria for mucosal HIV vaccine 172–173

intrabodies in anti-HIV research 580–581

live-attenuated Sabin poliovirus as AIDS vaccine vector 174

mucosal immune system and vaccine development 173

sexual transmission 171–172

use of poliovirus vectors in developing countries 174

Adeno-associated virus (AAV)

as gene delivery vector 11

biology 93–97

electron microscope image 94

life cycles 96

development of new vectors 103–105

immunological aspects 102–103

in vitro and in vivo gene transfer 99–102

production of recombinant vectors 97–99

Adenoviridae family 10

Adenovirus (Ad) 109–111, 122

as gene delivery vector 10

gene delivery

capsid proteins 115

cell entry pathway 115–116

cell receptors 115–116

conjugate targeting system 118

genetic targeting system 118–119

infection mechanisms 116

targeting 116–119

gene expression

high-level expression 119–120

specific transgene expression 120–121

tissue-specific promoters 120

tumor-specific promoters 120–121

genome 110

production and analysis of virus particles 121–122

vectors 110, 111

first generation 111–112

replication 114

second generation 112–113

third generation 113–114

Adult T-cell leukemia (ATL) 581

Aequorin 294

Affinity chromatography 649–650

fusion proteins 650–651

high-resolution chromatography 651–652

African green monkey CV1 kidney cell line (COS) 2, 9, 84

COS-7 cells 76, 77, 79, 84, 85, 215

African green monkey kidney cell line (Vero) 2

Alkaline phosphatase (AP) 303–304

Alpha fetoprotein (AFP) promoter 120

Alphaviruses 189–190

effects of alphaviruses and replicons on infected cells 193–194

future perpectives 202–203

Alzheimer's disease 582

Aminoglycoside phosphotransferase (neo) gene 274

(±)-N-(3-Aminopropyl-N,N-dimethyl-2,3-bis(dodecyloxy)-1-propanammonium bromide (GAP-DLRIE) 280, 281

Amphotropic envelope of MLV (Ampho) 259–260

Amplicons 35, 36

Amyloid precursor protein (APP) 216

Antibiotic-responsive systems 592

erythromycin-response gene expression (E.REX) 594–595

streptogramin-responsive gene expression (PIP systems) 593–594

tetracycline-responsive gene expression 592–593

...

Table of contents

• Cover image
• Title page
• Table of Contents
• Preface
• Why choose mammalian cells for protein production?
• Vectors for gene expression in mammalian cells
• Virus-based vectors for gene expression in mammalian cells: Herpes simplex virus
• Virus-based vectors for gene expression in mammalian cells: Epstein-Barr virus
• Virus-based vectors for gene expression in mammalian cells: SV40
• Virus-based vectors for gene expression in mammalian cells: Adeno-associated virus
• Virus-based vectors for gene expression in mammalian cells: Adenovirus
• Virus-based vectors for gene expression in mammalian cells: Vaccinia virus
• Virus-based vectors for gene expression in mammalian cells: Baculovirus
• Virus-based vectors for gene expression in mammalian cells: Coronavirus
• Virus-based vectors for gene expression in mammalian cells: Poliovirus
• Virus-based vectors for gene expression in mammalian cells: Sindbis virus
• Virus-based vectors for gene expression in mammalian cells: Semliki Forest virus
• Virus-based vectors for gene expression in mammalian cells: Retrovirus
• Virus-based vectors for gene expression in mammalian cells: Lentiviruses
• Methods for DNA introduction into mammalian cells
• Lipid reagents for DNA transfer into mammalian cells
• Reporter genes for monitoring gene expression in mammalian cells
• Gene transfer and gene amplification in mammalian cells
• Co-transfer of multiple plasmids/viruses as an attractive method to introduce several genes in mammalian cells
• Optimization of plasmid backbone for gene expression in mammalian cells
• Use of scaffold/matrix-attachment regions for protein production
• Chromatin insulators and position effects
• Locus control regions
• Protein synthesis, folding, modification, and secretion in mammalian cells
• Pathways and functions of mammalian protein glycosylation
• Metabolic engineering of mammalian cells for higher protein yield
• Translational regulation in mammalian cells
• Pathways of mammalian messenger RNA degradation
• Pathways of mammalian protein degradation
• Stabilization of proteasomal substrates by viral repeats
• Architecture and utilization of highly expressed genomic sites
• Intracellular targeting of antibodies in mammalian cells
• Inducible gene expression in mammalian cells
• Protein production by large-scale mammalian cell culture
• Protein production in transgenic animals
• Strategies for the purification of recombinant proteins
• Subject Index