Centrifugation in Density Gradients

C. A. Price

• Publisher: Academic Press
• Year: 2014
• ISBN:
  9781483282060
• Topic: Sciences biologiques
• Subtopic: Biologie

Centrifugation in Density Gradients provides information pertinent to the fundamental aspects of density gradient centrifugation. This book discusses the benefits of density gradient centrifugation to membrane-bound particles. Organized into nine chapters, this book begins with an overview of the method of differential or fractional centrifugation. This text then explores the physical basis of density gradient centrifugation. Other chapters deal with the nuts and bolts of density gradient centrifugation, the construction and composition of gradients, the properties and operation of centrifuge systems, and certain arcane but highly useful procedures. This book discusses as well density gradient centrifugation in the analytical ultracentrifuge. The final chapter deals with a collection of protocols for separating particles ranging in size from whole cells to macromolecules. This book is intended to be suitable for readers who need to separate biological particles. Biologists, chemists, biochemists, cytologists, physiologists, scientists, and research workers will also find this book useful.

Table of contents

• Cover image
• Title page
• Table of Contents
• Copyright
• Dedication
• Foreword
• Preface
• Chapter 1: Particle Abstraction in Biology—An Introduction
• Chapter 2: Origins of Density Gradient Centrifugation
• Chapter 3: Sedimentation Theory: A Semiquantitative Approach
• Chapter 4: Sedimentation Theory: A More Rigorous Approach
• Chapter 5: Gradient Materials and Construction of Gradients
• Chapter 6: Techniques of Preparative Density Gradient Centrifugation
• Chapter 7: Special Topics
• Chapter 8: Analytical Centrifugation in Density Gradients
• Chapter 9: Protocols for the Separation and Analysis of Some Specific Particles
• Appendix A: Some Useful Units, Values, and Conversions
• Appendix B: Properties of Particles
• Appendix C: Properties of Gradient Materials
• Appendix D: Gradient Shapes
• Appendix E: Zonal Rotors
• Appendix F: Chemical Resistance of Various Plastics
• Appendix G: Addresses of Some Manufacturers
• Index