Tissue Culture
eBook - ePub

Tissue Culture

Methods and Applications

Paul F. Jr. Kruse

  1. 896 pages
  2. English
  3. ePUB (mobile friendly)
  4. Available on iOS & Android
eBook - ePub

Tissue Culture

Methods and Applications

Paul F. Jr. Kruse

Book details
Table of contents
Citations

About This Book

Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.

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Information

Publisher
Academic Press
Year
2012
ISBN
9780323142076
Topic
Biological Sciences
Subtopic
Biology
Index
Biological Sciences

Table of contents

  1. Cover image
  2. Title page
  3. Table of Contents
  4. Copyright
  5. List of Contributors
  6. Preface
  7. Dr. Paul F. Kruse, Jr. (1921–1973)
  8. SECTION I: PRIMARY TISSUE DISSOCIATION
  9. SECTION II: PREPARATION OF PRIMARY CULTURES
  10. SECTION III: CELL HARVESTING
  11. SECTION IV: REPLICATE CULTURE METHODS
  12. SECTION V: SINGLE CELL ISOLATIONS AND CLONING
  13. SECTION VI: PERFUSION AND MASS CULTURE TECHNIQUES
  14. SECTION VII: CELL PROPAGATION ON MISCELLANEOUS CULTURE SUPPORTS
  15. SECTION VIII: EVALUATION OF CULTURE DYNAMICS
  16. SECTION IX: RECENT TECHNIQUES FACILITATING MICROSCOPIC OBSERVATION OF CELLS
  17. SECTION X: CELL HYBRIDIZATION
  18. SECTION XI: VIRUS PROPAGATION AND ASSAY
  19. SECTION XII: PRODUCTION OF HORMONES AND INTERCELLULAR SUBSTANCES
  20. SECTION XIII: DIAGNOSIS AND UNDERSTANDING OF DISEASE
  21. SECTION XIV: QUALITY CONTROL MEASURES
  22. Author Index
  23. Subject Index