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Tissue Culture
Methods and Applications
Paul F. Jr. Kruse
- 896 pages
- English
- ePUB (mobile friendly)
- Available on iOS & Android
Tissue Culture
Methods and Applications
Paul F. Jr. Kruse
About This Book
Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.
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Table of contents
- Cover image
- Title page
- Table of Contents
- Copyright
- List of Contributors
- Preface
- Dr. Paul F. Kruse, Jr. (1921â1973)
- SECTION I: PRIMARY TISSUE DISSOCIATION
- SECTION II: PREPARATION OF PRIMARY CULTURES
- SECTION III: CELL HARVESTING
- SECTION IV: REPLICATE CULTURE METHODS
- SECTION V: SINGLE CELL ISOLATIONS AND CLONING
- SECTION VI: PERFUSION AND MASS CULTURE TECHNIQUES
- SECTION VII: CELL PROPAGATION ON MISCELLANEOUS CULTURE SUPPORTS
- SECTION VIII: EVALUATION OF CULTURE DYNAMICS
- SECTION IX: RECENT TECHNIQUES FACILITATING MICROSCOPIC OBSERVATION OF CELLS
- SECTION X: CELL HYBRIDIZATION
- SECTION XI: VIRUS PROPAGATION AND ASSAY
- SECTION XII: PRODUCTION OF HORMONES AND INTERCELLULAR SUBSTANCES
- SECTION XIII: DIAGNOSIS AND UNDERSTANDING OF DISEASE
- SECTION XIV: QUALITY CONTROL MEASURES
- Author Index
- Subject Index