Tissue Culture

Methods and Applications

Paul F. Jr. Kruse

• Publisher: Academic Press
• Year: 2012
• Print ISBN: 9780124271500
• eBook ISBN: 9780323142076
• Topic: Biological Sciences
• Subtopic: Biology
• Index: Biological Sciences

Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.

Table of contents

• Cover image
• Title page
• Table of Contents
• Copyright
• List of Contributors
• Preface
• Dr. Paul F. Kruse, Jr. (1921–1973)
• SECTION I: PRIMARY TISSUE DISSOCIATION
• SECTION II: PREPARATION OF PRIMARY CULTURES
• SECTION III: CELL HARVESTING
• SECTION IV: REPLICATE CULTURE METHODS
• SECTION V: SINGLE CELL ISOLATIONS AND CLONING
• SECTION VI: PERFUSION AND MASS CULTURE TECHNIQUES
• SECTION VII: CELL PROPAGATION ON MISCELLANEOUS CULTURE SUPPORTS
• SECTION VIII: EVALUATION OF CULTURE DYNAMICS
• SECTION IX: RECENT TECHNIQUES FACILITATING MICROSCOPIC OBSERVATION OF CELLS
• SECTION X: CELL HYBRIDIZATION
• SECTION XI: VIRUS PROPAGATION AND ASSAY
• SECTION XII: PRODUCTION OF HORMONES AND INTERCELLULAR SUBSTANCES
• SECTION XIII: DIAGNOSIS AND UNDERSTANDING OF DISEASE
• SECTION XIV: QUALITY CONTROL MEASURES
• Author Index
• Subject Index