Methods of Enzymatic analysis V4

Hans-UIrich Bergmeyer

• Publisher: Academic Press
• Year: 2012
• ISBN:
  9780323161329
• Edition: 2
• Topic: Ciencias biológicas
• Subtopic: Bioquímica

Methods of Enzymatic Analysis, Volume 4 reviews developments in the use of enzymes as tools in analytical biochemistry, including advances in assay techniques. It discusses the principles and methods for the elucidation of structures of enzymes, such as peptides, proteins, amino acids, fatty acid metabolites, lipids, steroids, nucleic acids, purines, pyrimidines, nucleosides, and coenzymes. It also considers the isolation and characterization of active centers in enzymes. This volume is divided into four parts, each discussing a group of enzymes and their determination. Part I focuses on proteins, peptides, and amino acids including amines and amides. Part II is concerned with fatty acid metabolites, lipids, and steroids ranging from polyunsaturated fatty acids and lecithin to choline, acetylcholine, triglycerides, glycerol, acetoacetate, triacetate, fumarylacetoacetate, 20-ketosteroids, prostaglandins, bile acids, and cholesterol. Part III discusses nucleic acids, purines, pyrimidines, nucleosides, coenzymes, and related compounds, whereas Part IV looks at other substrates and effectors such as inorganic phosphate. The book concludes with a chapter on metabolites and their concentrations in animal tissues. Biochemists as well as students and researchers working in the field of analytical biochemistry will find this book highly informative.

Table of contents

• Cover image
• Title page
• Table of Contents
• Copyright
• Preface
• From the Preface to the 1st Edition
• Contributors
• Abbreviations of Units of Mass and Constants
• III: Proteins, Peptides and Amino Acids
• IV: Fatty Acid Metabolites, Lipids and Steroids
• V: Nucleic Acids, Purines, Pyrimidines, Nucleosides, Coenzymes and Related Compounds
• VI: Other Substrates and Effectors
• Concentrations of Metabolites in Animal Tissues
• Index
• Atomic Weights