Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols. Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, monclonal antibodies, and evolved proteins.

Prepared by experts in the field
Provides proven laboratory protocols, troubleshooting, and tips
Includes maps, sequences, and sample data
Contains extensive and up-to-date references

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Yes, you can access Phage Display of Peptides and Proteins by Brian K. Kay,Jill Winter,John McCafferty in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Molecular Biology. We have over one million books available in our catalogue for you to explore.

Information

Publisher

Academic Press

Year

1996

ISBN

9780080538709

Topic

Biological Sciences

Subtopic

Molecular Biology

Index

Biological Sciences

Cover image
Title page
Table of Contents
Copyright
CONTRIBUTORS
FOREWORD
PREFACE
Chapter 1: Biology of the Filamentous Bacteriophage
Chapter 2: Principles and Applications of Phage Display
Chapter 3: Vectors for Phage Display
Chapter 4: Microbiological Methods
Chapter 5: Construction of Random Peptide Libraries in Bacteriophage M13
Chapter 6: Construction and Screening of Antibody Display Libraries
Chapter 7: Phagemid-Displayed Peptide Libraries
Chapter 8: Multiple Display of Foreign Peptide Epitopes on Filamentous Bacteriophage Virions
Chapter 9: Phage Libraries Displaying Random Peptides Derived from a Target Sequence
Chapter 10: Display and Selection of Proteins on Genetic Packages
Chapter 11: A cDNA Cloning System Based on Filamentous Phage: Selection and Enrichment of Functional Gene Products by Protein/Ligand Interactions Made Possible by Linkage of Recognition and Replication Functions
Chapter 12: Multicombinatorial Libraries and Combinatorial Infection: Practical Considerations for Vector Design
Chapter 13: Screening Phage-Displayed Random Peptide Libraries
Chapter 14: Substrate Phage
Chapter 15: Phage Display: Factors Affecting Panning Efficiency
Chapter 16: Preparation of Second-Generation Phage Libraries
Chapter 17: Nonradioactive Sequencing of Random Peptide Recombinant Phage
Chapter 18: Measurement of Peptide Binding Affinities Using Fluorescence Polarization
APPENDIX
INDEX