Manual of Techniques in Insect Pathology

Lawrence A. Lacey

• Publisher: Academic Press
• Year: 1997
• ISBN:
  9780080535777
• Topic: Technology & Engineering
• Subtopic: Agriculture
• Index: Technology & Engineering

Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.
In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.
The books will be of value to advances researches and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.
This manual describes the broad array of techniques that are used in insect pathology. It will provide biologists, insect pathologists, entomologists, and those interested in biological control, with the necessary information to work on a variety of pathogen groups.
This book will be an essential laboratory reference for insect pathologists.Features include:
* Step by-step instructions on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens
* Details of the practical knowledge needed by beginners to apply the techniques
* Chapters written by an international group of experts
* Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques
* Provides extensive supplemental literature and recipes for media, fixatives and stains

Table of contents

• Front Cover
• Manual of Techniques in Insect Pathology
• Copyright Page
• Contents
• Series Preface
• Contributors
• Preface
• Acknowledgements
• Chapter 1. Initial handling and diagnosis of diseased insects
• Chapter II. Viruses
• Chapter III-1. Bacteria: Identification, isolation, culture and preservation of entomopathogenic bacteria
• Chapter III-2. Bacteria: Laboratory bioassay of bacteria against aquatic insects with emphasis on larvae of mosquitoes and black flies
• Chapter III-3. Bacteria: Bioassay of Bacillus thuringiensis against lepidopteran larvae
• Chapter III-4. Bacteria of soil-inhabiting insects
• Chapter IV. Research methods for entomopathogenic protozoa
• Chapter V-1. Fungi: Identification
• Chapter V-2. Fungi: Entomophthorales
• Chapter V-3. Fungi: Hyphomycetes
• Chapter V-4. Fungi: Oomyeetes and Chytridiomycetes
• Chapter V-5. Fungi: Preservation of cultures
• Chapter VI. Techniques in insect nematology
• Chapter VII. Testing the pathogenicity and infectivity of entomopathogens to mammals
• Chapter VIII- 1. Complementary techniques: Preparations of entomopathogens and diseased specimens for more detailed study using microscopy
• Chapter VIII-2. Complementary techniques: Fluorescence microscopy
• Chapter VIII-3. The application of molecular techniques to insect pathology with emphasis on entomopathogenic fungi
• Index
• Color Plate Section