In Vitro Methods in Cell-Mediated Immunity
eBook - ePub

In Vitro Methods in Cell-Mediated Immunity

  1. 602 pages
  2. English
  3. ePUB (mobile friendly)
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eBook - ePub

In Vitro Methods in Cell-Mediated Immunity

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About This Book

In Vitro Methods in Cell-Mediated Immunity focuses on methods for approaching cell-mediated immune responses in vitro. This book provides, in simplified in vitro systems, a basis for understanding the mechanism of the in vivo response and discusses useful and reliable in vitro tests for cell-mediated immune responses in humans, where in vivo testing is often not possible. The topics discussed include factors and activities produced in vitro by lymphocytes; biological implications of in vitro phenomena; and requirements and prospects for improved methodology. The leucocyte migration technique for in vitro detection of cellular hypersensitivity in man; proliferation of human blood lymphocytes stimulated by antigen in vitro; and virus plaque assay for antigen-sensitive are also elaborated in this text. This publication is a good reference for microbiologist and immunologists, including medical students researching on in vitro models for cell-mediated immune reactions.

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Yes, you can access In Vitro Methods in Cell-Mediated Immunity by Barry R. Bloom,Philip R. Glade in PDF and/or ePUB format, as well as other popular books in Biowissenschaften & Zellbiologie. We have over one million books available in our catalogue for you to explore.

Information

Publisher
Academic Press
Year
2014
ISBN
9781483277592
Topic
Biowissenschaften
Subtopic
Zellbiologie
SECTION B
METHODS

INTRODUCTION TO METHODS

EDITORSā€™ NOTE:

The individual methods are printed essentially as submitted by the authors. Except for minor changes to improve clarity, they were not edited or revised; the final copy was corrected by the authors themselves. The editors of this book cannot assume responsibility for inaccuracies in the text or difficulties encountered with individual methods.

INDEX OF ASSAYS AND SPECIES

Index of Assays And Species For Which They are Described
Human Guinea Pig Mouse Rat
CELL MIGRATION 5, 6, 7, 8, 9, 10 1, 2, 3, 4 9 9
Other effects on macrophages 11, 12 13
CYTOTOXICITY 14, 15, 17 14, 15, 16 14, 18
GROWTH INHIBITION 19, 20, 22, 23 21
BLASTOGENESIS 26, 27, 28, 29, 30 24 25
CHEMOTAXIS 31 31
INTERFERON 32, 33
SKIN REACTIVITY 1, 34
TRANSFER FACTOR 35, 36
VIRUS PLAQUE ASSAY 37
1

THE ASSAY OF INHIBITION OF MACROPHAGE MIGRATION AND THE PRODUCTION OF MIGRATION INHIBITORY FACTOR (MIF) AND SKIN REACTIVE FACTOR (SRF) IN THE GUINEA PIG*

Barry R. Bloom and Boyce Bennett

Publisher Summary

The capillary tube method for studying the inhibition of macrophage migration in vitro has been widely shown to be a useful correlate of delayed-type hypersensitivity in experimental animals. Recent reports indicate its potential usefulness in man. In animal studies, the technique has been successfully applied for the detection of transplantation antigens and cell-associated tumor antigens, as well as soluble tumor antigens. It has been shown that this reaction is mediated by a soluble factor, produced by sensitized lymphocytes upon stimulation by specific antigen, that is, migration inhibitory factor. Culture supernatants containing this factor...

Table of contents

  1. Cover image
  2. Title page
  3. Table of Contents
  4. Inside Front Cover
  5. Copyright
  6. PARTICIPANTS
  7. CONTRIBUTORS
  8. PREFACE
  9. INTRODUCTORY NOTE
  10. SECTION A: SESSIONS
  11. SECTION B: METHODS
  12. APPENDIX TABLES
  13. ABBREVIATIONS
  14. INDEX OF PARTICIPANTS