Pulsed Field Gel Electrophoresis
eBook - PDF

Pulsed Field Gel Electrophoresis

A Practical Guide

  1. 272 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

Pulsed Field Gel Electrophoresis

A Practical Guide

Book details
Table of contents
Citations

About This Book

Pulsed Field Gel Electrophoresis: A Practical Guide is the first laboratory manual to describe the theory and practice of this technique. Based on the authors' experience developing pulsed field gel instruments and teaching procedures, this book provides everything a researcher or student needs to know in order to understand and carry out pulsed field gel experiments. Clear, well-tested protocols assume only that users have a basic familiarity with molecular biology. Thorough coverage of useful data, theory, and applications ensures that this book is also a lasting resource for more advanced practitioners of pulsed field gels.

  • Reviews all types of pulsed field gel electrophoresis
  • Describes all commercially available systems and summarizes advantages and limitations of each
  • Includes step-by-step protocols for sample preparation and analysis
  • Illustrated with photographs that depict
  • How to run gels: What the results should look like
  • What they look like when they go wrong
  • Covers applications to a wide range of organisms
  • Includes bibliography of over 900 publications and cross-referenced by topic, application, and organism

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Yes, you can access Pulsed Field Gel Electrophoresis by Bruce Birren,Eric Lai in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Biology. We have over one million books available in our catalogue for you to explore.

Information

Publisher
Academic Press
Year
2012
ISBN
9780323137621
Topic
Biological Sciences
Subtopic
Biology
Index
Biological Sciences

Table of contents

  1. Front Cover
  2. Pulsed Field Gel Electrophoresis
  3. Copyright Page
  4. Table of Contents
  5. PREFACE
  6. CHAPTER 1. INTRODUCTION TO CONVENTIONAL AND PULSED FIELD GEL ELECTROPHORESIS
  7. CHAPTER 2. SETTING UP A PULSED FIELD GEL ELECTROPHORESIS SYSTEM
  8. CHAPTER 3. PREPARATION OF DNA FOR PULSED FIELD ANALYSIS
  9. CHAPTER 4. POURING GELS AND HANDLIN G SAMPLES
  10. CHAPTER 5. RESTRICTION ENZYME DIGESTION OF LARGE DNA FRAGMENT SINAGAROSE
  11. CHAPTER 6. SWITCH INTERVALS A N D RESOLUTION IN PULSED FIELD GELS
  12. CHAPTER 7. FIELD INVERSION GEL ELECTROPHORESIS
  13. CHAPTER 8. VARIABLES THAT AFFECT PULSED FIELD GELS
  14. CHAPTER 9. SOUTHERN BLOT ANALYSIS OF PULSED FIELD GELS
  15. CHAPTER 10. SPECIAL APPLICATIONS
  16. CHAPTER 11. TROUBLESHOOTING
  17. Appendix A: SCHEMATIC DIAGRAM OF THE CHEF SYSTEM
  18. Appendix B: SCHEMATIC DIAGRAM OF THE FIGE SYSTEM
  19. Appendix C: SCHEMATIC DIAGRAM OF THE ELECTRICAL CIRCUIT FOR AVFIGE
  20. BIBLIOGRAPHY OF PULSED FIELD GEL ELECTROPHORESIS
  21. INDEX