Pulsed Field Gel Electrophoresis

A Practical Guide

Bruce Birren, Eric Lai

• Publisher: Academic Press
• Year: 2012
• Print ISBN:
  9780121012908
• eBook ISBN:
  9780323137621
• Topic: Biological Sciences
• Subtopic: Biology
• Index: Biological Sciences

Pulsed Field Gel Electrophoresis: A Practical Guide is the first laboratory manual to describe the theory and practice of this technique. Based on the authors' experience developing pulsed field gel instruments and teaching procedures, this book provides everything a researcher or student needs to know in order to understand and carry out pulsed field gel experiments. Clear, well-tested protocols assume only that users have a basic familiarity with molecular biology. Thorough coverage of useful data, theory, and applications ensures that this book is also a lasting resource for more advanced practitioners of pulsed field gels. - Reviews all types of pulsed field gel electrophoresis - Describes all commercially available systems and summarizes advantages and limitations of each - Includes step-by-step protocols for sample preparation and analysis - Illustrated with photographs that depict - How to run gels: What the results should look like - What they look like when they go wrong - Covers applications to a wide range of organisms - Includes bibliography of over 900 publications and cross-referenced by topic, application, and organism

Table of contents

• Front Cover
• Pulsed Field Gel Electrophoresis
• Copyright Page
• Table of Contents
• PREFACE
• CHAPTER 1. INTRODUCTION TO CONVENTIONAL AND PULSED FIELD GEL ELECTROPHORESIS
• CHAPTER 2. SETTING UP A PULSED FIELD GEL ELECTROPHORESIS SYSTEM
• CHAPTER 3. PREPARATION OF DNA FOR PULSED FIELD ANALYSIS
• CHAPTER 4. POURING GELS AND HANDLIN G SAMPLES
• CHAPTER 5. RESTRICTION ENZYME DIGESTION OF LARGE DNA FRAGMENT SINAGAROSE
• CHAPTER 6. SWITCH INTERVALS A N D RESOLUTION IN PULSED FIELD GELS
• CHAPTER 7. FIELD INVERSION GEL ELECTROPHORESIS
• CHAPTER 8. VARIABLES THAT AFFECT PULSED FIELD GELS
• CHAPTER 9. SOUTHERN BLOT ANALYSIS OF PULSED FIELD GELS
• CHAPTER 10. SPECIAL APPLICATIONS
• CHAPTER 11. TROUBLESHOOTING
• Appendix A: SCHEMATIC DIAGRAM OF THE CHEF SYSTEM
• Appendix B: SCHEMATIC DIAGRAM OF THE FIGE SYSTEM
• Appendix C: SCHEMATIC DIAGRAM OF THE ELECTRICAL CIRCUIT FOR AVFIGE
• BIBLIOGRAPHY OF PULSED FIELD GEL ELECTROPHORESIS
• INDEX