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Diatom Microscopy
About This Book
DIATOM MICROSCOPY
The main goal of the book is to demonstrate the wide variety of microscopy methods being used to investigate natural and altered diatom structures.
This book on Diatom Microscopy gives an introduction to the wide panoply of microscopy methods being used to investigate diatom structure and biology, marking considerable advances in recent technology including optical, fluorescence, confocal and electron microscopy, surface-enhanced Raman spectroscopy (SERS), atomic force microscopy (AFM) and spectroscopy as applied to diatoms. Each chapter includes a tutorial on a microscopy technique and reviews its applications in diatom nanotechnology and diatom research. The number of diatomists, diatom research, and their publications are increasing rapidly. Although many books have dealt with various aspects of diatom biotechnology, nanotechnology, and morphology, to our knowledge, no volume exists that summarizes advanced microscopic approaches to diatoms.
Audience
The intended audience is academic and industry researchers as well as graduate students working on diatoms and diatom nanotechnology, including biosensors, biomedical engineering, solar panels, batteries, drug delivery, insect control, and biofuels.
Frequently asked questions
Information
1
Investigation of Diatoms with Optical Microscopy
AbstractDiatoms are eukaryotic microalgae occurring in the water column as phytoplankton and on the ocean bed as benthic microalgae. Diatoms are similar to plants in the fact that they use photosynthesis, but differ in terms of evolutionary classification. More than 10,000 diatom species have been formally described, based primarily on the unique patterning of their silica shells. Over the last two decades, diatoms have attracted considerable attention for their potential use in synthesizing bio-functional materials due to the advantages of low production cost, prevention of using toxic chemicals and producing hazardous waste and intricate 3D hierarchical structures applicable for photonic applications, biosensors, catalysis, adsorption, and drug delivery systems. All such applications require a comprehensive understanding of the diatom structure, from the microscale to the nanoscale. Optical imaging provides spatial resolution at the sub-micrometer scale without harming the specimens. Image post-processing and reconstruction also make it possible to render the structure of samples in 3D via optical sectioning. In this chapter, we explore the various facets of optical microscopy within the context of diatom research and the applicability of this work to eco-environmental science and biomedicine. In the following sections, we respectively address light microscopy, fluorescence microscopy, confocal laser scanning microscopy, multiphoton microscopy, and super-resolution optical microscopy.Keywords: Light microscopy, phase contrast microscopy, differential inteference contrast microscopy, darkfield microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy, super-resolution optical microscopy
1.1 Introduction
Table of contents
- Cover
- Table of Contents
- Title Page
- Copyright
- Preface
- 1 Investigation of Diatoms with Optical Microscopy
- 2 Nanobioscience Studies of Living Diatoms Using Unique Optical Microscopy Systems
- 3 Recent Insights Into the Ultrastructure of Diatoms Using Scanning and Transmission Electron-Microscopy
- 4 Atomic Force Microscopy Study of Diatoms
- 5 Refractive Index Tomography for Diatom Analysis
- 6 Luminescent Diatom Frustules: A Review on the Key Research Applications
- 7 Micro to Nano Ornateness of Diatoms from Geographically Distant Origins of the Globe
- 8 Types of X-Ray Techniques for Diatom Research
- 9 Diatom Assisted SERS
- 10 Diatoms as Sensors and Their Applications
- 11 Diatom Frustules: A Transducer Platform for Optical Detection of Molecules
- 12 Effects of Light on Physico-Chemical Properties of Diatoms
- Index
- Also of Interest
- End User License Agreement