Bacterial Sensors
eBook - PDF

Bacterial Sensors

Synthetic Design and Application Principles

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eBook - PDF

Bacterial Sensors

Synthetic Design and Application Principles

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About This Book

Bacterial reporters are live, genetically engineered cells with promising application in bioanalytics. They contain genetic circuitry to produce a cellular sensing element, which detects the target compound and relays the detection to specific synthesis of so-called reporter proteins (the presence or activity of which is easy to quantify). Bioassays with bacterial reporters are a useful complement to chemical analytics because they measure biological responses rather than total chemical concentrations. Simple bacterial reporter assays may also replace more costly chemical methods as a first line sample analysis technique. Recent promising developments integrate bacterial reporter cells with microsystems to produce bacterial biosensors.This lecture presents an in-depth treatment of the synthetic biological design principles of bacterial reporters, the engineering of which started as simple recombinant DNA puzzles, but has now become a more rational approach of choosing and combining sensing, controlling and reporting DNA 'parts'. Several examples of existing bacterial reporter designs and their genetic circuitry will be illustrated. Besides the design principles, the lecture also focuses on the application principles of bacterial reporter assays. A variety of assay formats will be illustrated, and principles of quantification will be dealt with. In addition to this discussion, substantial reference material is supplied in various Annexes.Table of Contents: Short History of the use of Bacteria for Biosensing and Bioreporting / Genetic Engineering Concepts / Measuring with Bioreporters / Epilogue

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Yes, you can access Bacterial Sensors by Jan Roelof Meer in PDF and/or ePUB format, as well as other popular books in Mathematics & Mathematics General. We have over one million books available in our catalogue for you to explore.

Information

Publisher
Springer
Year
2022
ISBN
9783031025709
Topic
Mathematics
Subtopic
Mathematics General
Index
Mathematics

Table of contents

  1. Cover
  2. Copyright Page
  3. Title Page
  4. Contents
  5. Preface
  6. Acknowledgments
  7. 1 Short History of the use of Bacteria for Biosensing and Bioreporting
  8. 2 Genetic Engineering Concepts
  9. 3 Measuring with Bioreporters
  10. 4 Epilogue
  11. A Bacterial Bioreporter DesignsTargeting Organic Compounds
  12. B Bacterial Bioreporter DesignsTargeting (Heavy) Metals and Metalloids
  13. C Bacterial Bioreporter Designs Responsive toToxicity or Stress Conditions
  14. D Example Bioreporter Protocols
  15. Author’s Biography