A Practical Guide to Scanning Electron Microscopy in the Biosciences

Gerhard Wanner

• Publisher: Wiley-VCH
• Year: 2022
• ISBN:
  9783527838479
• Edition: 1
• Topic: Physical Sciences
• Subtopic: Science History & Practice

A concise and authoritative introduction to scanning electron microscopy in the biological sciences

In A Practical Guide to Scanning Electron Microscopy distinguished electron microscopist Gerhard Wanner delivers a practical handbook for biological scientists working with microbial, plant, and animal cells and tissues, enabling them to successfully apply scanning electron microscopy (SEM) to their object of study.

The book begins with an introduction to the principles of electron microscopy and the operation of electron microscopes before moving on to describe the preparation and mounting of specimens. It also explores the process of recoding images and their subsequent analysis, along with a wide range of advanced microscopy techniques, including cryo-SEM, FIB-SEM tomography, and stereo-SEM.

Scanning Electron Microscopy in the Biosciences contains hundreds of carefully selected microscopic images, as well as hands-on, step-by-step guidance required to perform a successful TEM experiment. Readers will also find:

• Thorough introductions to optics, electron microscopy, electrons, and the components of electron microscopes
• In-depth examinations of the preparation of biological specimens and specimen mounting for scanning electron microscopy
• A comparison of different SEM modes and their strengths and weaknesses
• An introduction to novel techniques such as correlative light and electron microscopy (CLEM), array tomography, and cryo-scanning electron microscopy

Perfect for cell biologists and microbiologists, A Practical Guide to Scanning Electron Microscopy in the Biosciences also belongs in the libraries of neurobiologists and biophysicists.

Table of contents

• Cover
• Title Page
• Copyright Page
• Dedication Page
• Foreword
• Content
• Frequently recurring abbreviations
• Chapter 1 Introduction
• Chapter 2 Optics
• Chapter 3 Electron microscopy
• Chapter 4 Electrons
• Chapter 5 Components of the EM
• Chapter 6 Preparation of biological samples
• Chapter 7 Specimen mounting for SEM
• Chapter 8 CLEM
• Chapter 9 Microscopy
• Chapter 10 Resolution
• Chapter 11 Analytics
• Chapter 12 EDX
• Chapter 13 FIB/SEM
• Chapter 14 Serial block face SEM (SBF)
• Chapter 15 Array tomography
• Chapter 16 Cryo-SEM
• Chapter 17 VP–SEM
• Chapter 18 Stereo SEM
• Glossary
• References
• Index
• EULA