Concepts and Models for Drug Permeability Studies

Cell and Tissue based In Vitro Culture Models

Bruno Sarmento, Catarina Leite Pereira, José Das Neves

• Publisher: Woodhead Publishing
• Year: 2024
• ISBN:
  9780443155116
• Edition: 2
• Topic: Medicine
• Subtopic: Pharmacology

Concepts and Models for Drug Permeability Studies: Cell and Tissue Based in Vitro Culture Models, Second Edition, summarizes the most important developments in in vitro models for predicting the permeability of drugs. This book is structured around three different approaches, summarizing the most recent achievements regarding models comprising (i) immortalized cells with an intrinsic ability to grow as monolayers when seeded in permeable supports, (ii) primary cells isolated from living organisms and directly cultured as barrier monolayers, and (iii) tissue-based models constructed with cell lines and extracellular matrix that resembles the tridimensional structure of mucosae and other biological membranes, or animal/patient-derived tissues. Each model is covered in detail, including the protocol of generation and application for specific drugs/drug delivery systems. The equivalence between in vitro cell and tissue models and in vivo conditions is discussed, highlighting how each model may provisionally resemble different drug absorption route. Chapters included in the first edition were updated with relevant data published in recent years, while four new chapters were included to reflect new emerging directions and trends in drug permeability models.

Concepts and Models for Drug Permeability Studies: Cell and Tissue Based in Vitro Culture Models, Second Edition, is a critical reference for drug discovery and drug formulation scientists interested in delivery systems intended for the administration of drugs through mucosal routes and other important tissue barriers (e.g. the BBB). Researchers studying mucosal biology can use this book to familiarize themselves and exploit the synergic effect of mucosal delivery systems and biomolecules.

• Summarizes the current advances in the use of permeability models in drug transport
• Covers the most important buccal, gastric, intestinal, pulmonary, nasal, vaginal, ocular, renal, skin, and blood–brain barrier in vitro models. Includes case studies to facilitate understanding of various concepts in computer-aided applications
• Updates in the second edition include organ-on-chip devices, 3D advanced models (multiple layered tissues, organoids, etc.), and multicompartmentalized tissue models

Table of contents

• Cover image
• Title page
• Table of Contents
• Copyright
• Contributors
• About the editors
• Chapter 1. Introduction
• Chapter 2. Importance and applications of cell- and tissue-based in vitro models for drug permeability screening in early stages of drug development
• Section I. Cell-based in vitro culture models for drug permeability studies: Immortalized and primary isolated cells
• Section II. Tissue-based in vitro and ex vivo models for drug permeability studies
• Section III. Miniaturized models for drug permeability studies
• Section IV. Complex models for drug permeability studies
• Index