Diagnostic Molecular Biology

Chang-Hui Shen

• Publisher: Academic Press
• Year: 2023
• ISBN:
  9780323986090
• Edition: 2
• Topic: Biological Sciences
• Subtopic: Molecular Biology
• Index: Biological Sciences

Diagnostic Molecular Biology, Second Edition describes the fundamentals of molecular biology in a clear, concise manner with each technique explained within its conceptual framework and current applications of clinical laboratory techniques comprehensively covered. This targeted approach covers the principles of molecular biology, including basic knowledge of nucleic acids, proteins and chromosomes; the basic techniques and instrumentations commonly used in the field of molecular biology, including detailed procedures and explanations; and the applications of the principles and techniques currently employed in the clinical laboratory. Topics such as whole exome sequencing, whole genome sequencing, RNA-seq, and ChIP-seq round out the discussion.

Fully updated, this new edition adds recent advances in the detection of respiratory virus infections in humans, like influenza, RSV, hAdV, hRV but also corona. This book expands the discussion on NGS application and its role in future precision medicine.

• Provides explanations on how techniques are used to diagnosis at the molecular level
• Explains how to use information technology to communicate and assess results in the lab
• Enhances our understanding of fundamental molecular biology and places techniques in context
• Places protocols into context with practical applications
• Includes extra chapters on respiratory viruses (Corona)

Table of contents

• Cover image
• Title page
• Table of Contents
• Copyright
• Dedication
• Preface
• Acknowledgement
• Chapter 1. Nucleic acids: DNA and RNA
• Chapter 2. Nucleic acid-based cellular activities – DNA replication, damage, and repair
• Chapter 3. Gene expression: transcription of the genetic code
• Chapter 4. Gene expression: translation of the genetic code
• Chapter 5. The genome
• Chapter 6. Extraction and purification of nucleic acids
• Chapter 7. Quantification and analysis of nucleic acids
• Chapter 8. Extraction and purification of proteins
• Chapter 9. Quantification and analysis of proteins
• Chapter 10. Amplification of nucleic acids
• Chapter 11. Characterization of nucleic acids and proteins
• Chapter 12. Techniques in sequencing
• Chapter 13. Genome and transcriptome analysis
• Chapter 14. Molecular diagnosis of chromosomal disorders
• Chapter 15. Molecular diagnosis of gene mutation and inherited diseases
• Chapter 16. Molecular diagnosis of infectious diseases
• Chapter 17. The biology of SARS-CoV-2 and the molecular diagnosis of COVID-19
• Chapter 18. Guidance for molecular diagnostic laboratory
• Bibliography
• Index