Subnuclear Components
eBook - PDF

Subnuclear Components

Preparation and Fractionation

  1. 348 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

Subnuclear Components

Preparation and Fractionation

Book details
Table of contents
Citations

About This Book

Subnuclear Components: Preparation and Fractionation focuses on the isolation of subnuclear components of eukaryotic cells. The book first discusses the isolation of nuclear envelopes from whole tissues. Topics include nuclear envelope in situ; general consideration and evaluation of isolation methods; and nuclear envelope isolation methods. The text describes the isolation of nucleoli, including the isolation of nucleoli from oocytes, nuclei, and physarum, and isolation of plant nucleoli. The book focuses on the preparation, characterization, and fractionation of chromatin. Emphasis is on the preparation of chromatin from interphase and metaphase cells; contaminants of chromatin; characteristics of isolated chromatin; and dissociation and reassociation of chromatin. The text also discusses fractionation and isolation of histones, including the preparation of individual histone fractions and isolation and purification of the five main histone fractions of calf thymus during one preparation. The book also looks at the preparation and properties of chromatin non-histone proteins, including isolation of nuclear RNA and isolation of DNA from eukaryotic cells. The selection is a good source of data for readers interested in the isolation of subnuclear constituents of eukaryotic cells.

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Yes, you can access Subnuclear Components by G. D. Birnie in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Biology. We have over one million books available in our catalogue for you to explore.

Information

Publisher
Butterworth-Heinemann
Year
2016
ISBN
9781483103402
Topic
Biological Sciences
Subtopic
Biology
Index
Biological Sciences

Table of contents

  1. Front Cover
  2. Subnuclear Components: Preparation and Fractionation
  3. Copyright Page
  4. Table of Contents
  5. Preface
  6. Contributors
  7. CHAPTER 1. ISOLATION OF ANIMAL CELL NUCLEI
  8. CHAPTER 2. ISOLATION OF NUCLEAR ENVELOPES FROM WHOLETISSUE
  9. CHAPTER 3. ISOLATION OF NUCLEOLI
  10. CHAPTER 4. PREPARATION, CHARACTERIZATION ANDFRACTIONATION OF CHROMATIN
  11. CHAPTER 5. FRACTIONATION AND ISOLATION OF HISTONES
  12. CHAPTER 6. PREPARATION AND PROPERTIES OF CHROMATINNON-HISTONE PROTEINS
  13. CHAPTER 7. ISOLATION OF NUCLEAR RNA
  14. CHAPTER 8. ISOLATION OF DNA FROM EUCARYOTIC CELLS
  15. INDEX