- 390 pages
- English
- PDF
- Available on iOS & Android
Maximizing Gene Expression
About This Book
Maximizing Gene Expression focuses on prokaryotic and eukaryotic gene expression. The book first discusses E. coli promoters. Topics include structure analysis, steps in transcription initiation, structure-function correlation, and regulation of transcription initiation. The text also highlights yeast promoters, including elements that select initiation sites, transcription regulation, regulatory proteins, and upstream promoter elements. The text also describes protein coding genes of higher eukaryotes; instability of messenger RNA in bacteria; and replication control of the ColE1-type plasmids. The text then describes translation initiation, including the translation of prokaryotes and eukaryotes. The book puts emphasis on the selective degradation of abnormal proteins in bacteria. Topics include proteins rapidly hydrolyzed in E. coli; intracellular aggregates of abnormal polypeptides; energy requirement and pathway for proteins; proteolytic enzymes in E. coli; and regulation of ion expression. The text also highlights the detection of proteins produced by recombinant DNA techniques and mechanism and practice. The book is a good source of information for readers wanting to study gene expression.
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Table of contents
- Front Cover
- Maximizing Gene Expression
- Copyright Page
- Table of Contents
- Contributors
- Preface
- Chapter 1. E. Coli Promoters
- Chapter 2. Yeast Promoters
- Chapter 3. Protein Coding Genes of Higher Eukaryotes: Promoter Elements and trans-Acting Factors
- Chapter 4. The Instability of Messenger RNA in Bacteria
- Chapter 5. Replication Control of the ColE1-Type Plasmids
- Chapter 6. Copy Number and Stability of Yeast Plasmids
- Chapter 7. Translation Initiation
- Chapter 8. Biased Codon Usage: An Exploration of Its Role in Optimization of Translation
- Chapter 9. The Selective Degradation of Abnormal Proteins in Bacteria
- Chapter 10. Detection of Proteins Produced by Recombinant DNA Techniques
- Chapter 11. Mechanism and Practice
- Index