The Separation and Isolation of Proteins
eBook - PDF

The Separation and Isolation of Proteins

a Laboratory Manual of Analytical Methods of Protein Chemistry (Including Polypeptides)

  1. 262 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

The Separation and Isolation of Proteins

a Laboratory Manual of Analytical Methods of Protein Chemistry (Including Polypeptides)

Book details
Table of contents
Citations

About This Book

A Laboratory Manual of Analytical Methods of Protein Chemistry (Including Polypeptides), Volume 1: The Separation and Isolation of Proteins deals with the techniques used in the separation and isolation of proteins, including fractionation and characterization by dialysis, multi-membrane electrodecantation, and zonal density gradient electrophoresis. The fractionation of proteins by adsorption and ion exchange is also described. This book is comprised of seven chapters and begins with a discussion on procedures for the separation of proteins, paying particular attention to the liberation of proteins from cellular material; removal of lipids from lipoproteins; and denaturation, fractionation, and purification of proteins. The next chapter focuses on the isolation of biologically active proteins such as cytochrome, bacterial amylases, and bacterial proteinases. The reader is methodically introduced to fractionation of proteins by adsorption and ion exchange; fractionation and characterization by dialysis; multi-membrane electrodecantation; and continuous and discontinuous partition. The final chapter explains how zonal density gradient electrophoresis works as a separation method for natural mixtures of proteins, their degradation products, and other substances carrying electric charges in solution or suspension. This volume will be of interest to chemists working with proteins.

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Information

Publisher
Pergamon
Year
2014
ISBN
9781483149806
Topic
Physical Sciences
Subtopic
Organic Chemistry

Table of contents

  1. Front Cover
  2. The Separation and Isolation of Proteins
  3. Copyright Page
  4. Table of Contents
  5. PREFACE
  6. CONTENTS LIST: VOLUME 2
  7. CONTENTS LIST: VOLUME 3
  8. Chapter 1. SEPARATION OF PROTEINS
  9. Chapter 2. ISOLATION OF BIOLOGICALLY ACTIVE PROTEINS
  10. Chapter 3. FRACTIONATION OF PROTEINS BY ADSORPTION AND ION EXCHANGE
  11. Chapter 4. FRACTIONATION AND CHARACTERIZATION BY DIALYSIS
  12. Chapter 5. PARTITION
  13. Chapter 6. MULTI-MEMBRANE ELECTRODECANTATION
  14. Chapter 7. ZONAL DENSITY GRADIENT ELECTROPHORESIS
  15. AUTHOR INDEX
  16. SUBJECT INDEX