Gel Electrophoresis of Proteins
eBook - PDF

Gel Electrophoresis of Proteins

  1. 422 pages
  2. English
  3. PDF
  4. Only available on web
eBook - PDF

Gel Electrophoresis of Proteins

Book details
Table of contents
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About This Book

Gel Electrophoresis of Proteins focuses on the techniques, methodologies, reactions, and approaches involved in gel electrophoresis of proteins. The selection first covers steady-state gel electrophoresis systems and one-dimensional PAA-gel electrophoretic techniques to separate functional and denatured proteins. Discussions focus on affinity electrophoresis, structure and physico-chemical properties of polyacrylamide gels, moving boundary electrophoresis, isotachophoresis, fundamental steady-state electrophoresis systems, and fundamental properties of steady-state electrophoresis systems. The text then reviews conventional isoelectric focusing and immobilized pH gradients and high resolution two-dimensional polyacrylamide-gel electrophoresis. Topics include production of narrow pH gradients, extended pH gradients, polymerization kinetics, IEF in agarose matrices, titration curves, two-dimensional electrophoresis under non-denaturing conditions, and IEF in polyacrylamide matrices. The book tackles quantifying patterns from two-dimensional PAGE, protein staining and detection methods, and immunoelectrophoretic methods. Concerns include post-electrophoretic organic protein stains, silver and enzyme stains, detection of radioactive proteins, and programming languages. The selection is highly recommended for researchers wanting to conduct studies on gel electrophoresis of proteins.

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Yes, you can access Gel Electrophoresis of Proteins by Michael J Dunn in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Molecular Biology. We have over one million books available in our catalogue for you to explore.

Information

Publisher
Butterworth-Heinemann
Year
2014
ISBN
9781483193540
Topic
Biological Sciences
Subtopic
Molecular Biology
Index
Biological Sciences

Table of contents

  1. Front Cover
  2. Gel Electrophoresis of Proteins
  3. Copyright Page
  4. Table of Contents
  5. Preface
  6. List of Contributors
  7. List of Abbreviations
  8. Chapter 1. Steady-state Gel Electrophoresis Systems
  9. Chapter 2. One-dimensional PAA-gel Electrophoretic Techniques to Separate Functional and Denatured Proteins
  10. Chapter 3. Conventional Isoelectric Focusing and Immobilised pH Gradients
  11. Chapter 4. High Resolution Two-dimensional Polyacrylamide-gel Electrophoresis
  12. Chapter 5. Immunoelectrophoretic Methods
  13. Chapter 6. In situ Peptide Mapping of Proteins and Polypeptides Separated by Polyacrylamide Gel Electrophoresis
  14. Chapter 7. Protein Staining and Detection Methods
  15. Chapter 8. Quantifying Patterns from Two-dimensional PAGE
  16. Index