- 566 pages
- English
- PDF
- Only available on web
About This Book
Cell Biology: A Laboratory Handbook, Volume 3 is a handbook on cell biology and covers topics ranging from transfer of macromolecules and small molecules to cloning of embryos, transgenics, and gene targeting. Cell-free extracts, permeabilized cell systems, and expression systems are also discussed, along with proteins. Comprised of 58 chapters, this volume begins with a detailed account of microinjection of RNA, DNA, and proteins into somatic cells, followed by an analysis of computer-automated capillary microinjection of macromolecules into living cells. The reader is then introduced to syringe loading as a method for inserting macromolecules into cells in suspension; electroporation of cells; and the use of liposomes in drug targeting. Subsequent chapters focus on the cloning of rabbit embryos by nuclear transplantation; gene targeting by homologous recombination in embryonic stem cells; production and isolation of recombinant viruses; and gel electrophoresis. This book will be of interest to geneticists and molecular biologists.
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Table of contents
- Front Cover
- Cell Biology: A Laboratory Handbook
- Copyright Page
- CONTENTS OF OTHER VOLUMES
- CONTRIBUTORS TO VOLUME 3
- PART 11: TRANSFER OF MACROMOLECULES AND SMALL MOLECULES
- PART 12: CLONING OF EMBRYOS, TRANSGENICS, AND GENE TARGETING
- PART 13: CELL-FREEEXTRACTS, PERMEABILIZEDCELL SYSTEMS, AND EXPRESSION SYSTEMS
- PART 14: PROTEINS
- PART 15: APPENDICES
- LIST OF SUPPLIERS
- INDEX