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The Interpretation of Ultrastructure
About This Book
The Interpretation of Ulstrastructure is a collection of papers dealing with techniques in ulstrastructural research such as in fixation, reversed staining, ultrastructure in embryos and in plants, control of observations by light microscopy, and cytochemistry at the electron microscope level. One paper describes various fixatives used on rat liver tissue including a standard osmium tetroxide solution, pure melted osmium tetroxide, and strong osmium tetroxide solutions in carbon tetrachloride. Another paper points out that the use of electron micrographs of the cell nucleus and chromosomes should include approaches to chromosome analysis. For the investigator studying the three dimensional organization of chromosomes, his approaches should include classical cytology and cytogenetics, chemical and cytochemical studies on nuclei, as well as chemical and physical analysis of nucleic acid and nucleoprotein molecules. Several papers discuss the study of natural and artificial DNA-plasma by thin sections, an assessment of negative staining techniques for revealing ultrastructure, and other approaches in the study of biological ultrastructure by high-resolution electron microscopy. The collection will benefit microbiologists, biotechnologists, and academicians connected with the biological sciences.
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Table of contents
- Front Cover
- The Interpretation of Ultrastructure
- Copyright Page
- Table of Contents
- LIST OF CONTRIBUTORS
- PREFACE
- CHAPTER 1. CHEMICAL FIXATIVES FOR ELECTRON MICROSCOPY
- CHAPTER 2. WATER-SOLUBLE EMBEDDING MEDIA FOR ULTRASTRUCTURAL CYTOCHEMISTRY
- CHAPTER 3. CRITICAL EVALUATION OF ULTRASTRUCTURAL PATTERNS WITH RESPECT TO FIXATION
- CHAPTER 4. INTERPRETATION OF ULTRASTRUCTURE IN THE CELL NUCLEUS
- CHAPTER 5. CORRELATION OF ELECTRON MICROSCOPE AND X-RAY DIFFRACTION DATA IN ULTRASTRUCTURE STUDIES OF LIPOPROTEIN MEMBRANE SYSTEMS
- CHAPTER 6. QUANTITATIVE ELECTRON MICROSCOPY
- CHAPTER 7. CYTOLOGY AND MICROCYTOLOGY OF LIVING AND FIXED CYTOPLASMIC CONSTITUENTS IN THE EGGS OF TUBIFEX AND THE CELL OF AMOBEA PROTEUS
- CHAPTER 8. LIGHT AND ELECTRON MICROSCOPIC STUDY OF MEMBRANOUS CYTOPLASMIC ORGANELLES
- CHAPTER 9. NUCLEOSIDEPHOSPHATASE ACTIVITIES OF CYTOMEMBRANES
- CHAPTER 10. COMBINATION OF CYTOCHEMICAL STAINING METHODS FOR ENZYME LOCALIZATION WITH ELECTRON MICROSCOPY
- CHAPTER 11. NUCLEOPROTEIN LOCALIZATION IN ELECTRON MICROGRAPHS: METAL BINDING AND RADIOAUTOGRAPHY
- CHAPTER 12. THE STUDY OF NATURAL AND ARTIFICIAL DNA-PLASMS BY THIN SECTIONS
- CHAPTER 13. NEGATIVE STAINING AND ITS USE IN THE STUDY OF VIRUSES AND THEIR SEROLOGICAL REACTIONS
- CHAPTER 14. AN ASSESSMENT OF NEGATIVE STAINING TECHNIQUES FOR REVEALING ULTRASTRUCTURE
- CHAPTER 15. THE FINER STRUCTURE OF THE MITOTIC APPARATUS
- CHAPTER 16. INTERPRETATION OF THE ULTRATEXTURE IN GROWING PLANT CELL WALLS
- CHAPTER 17. THE ELECTRON MICROSCOPY AND ELECTRON DIFFRACTION ANALYSIS OF NATURAL CELLULOSE
- CHAPTER 18. THE MOLECULAR STRUCTURE OF LIPID-WATER SYSTEMS AND CELL MEMBRANE MODELS STUDIED WITH THE ELECTRON MICROSCOPE
- CHAPTER 19. THE EVOLUTION OF INTRACELLULAR PHOSPHOLIPID MEMBRANE SYSTEMS
- CHAPTER 20. RECOVERY OF VIRUS FROM THE BLOOD OF RATS WITH INDUCED LEUKAEMIA
- CHAPTER 21. ELECTRON MICROSCOPY IN THE STUDY OF EMBRYONIC DIFFERENTIATION
- CHAPTER 22. NEW APPROACHES IN THE STUDY OF BIOLOGICAL ULTRASTRUCTURE BY HIGH-RESOLUTI ON ELECTRON MICROSCOPY
- AUTHOR INDEX