Techniques for Biochemical Analysis

Khursheed Hussain,Sameena Maqbool Lone,Khalid Z. Masoodi,Syed Minu Balkhi

• Verlag: Academic Press
• Jahr: 2024
• ISBN:
  9780443159152
• Thema: Ciencias biológicas
• Thema: Biofísica

Techniques for Biochemical Analysis provides researchers with a practical guide for investigating a variety of different biomolecules. It includes a range of tried and tested protocols, outlining the principles upon which each technique is founded, as well as providing instructions on equipment setup and use, buffer preparation, reagents required, safety considerations and analysis of findings. Beginning with an introduction to biochemistry and laboratory procedures, the book moves on to specific methods focused on investigation of carbohydrates, proteins, enzymes, plant hormones, minerals, amino acids, and more. The large range of protocols covered in this foundational, how-to reference are interdisciplinary and adaptable to a variety of areas, making this an ideal resource for researchers across various fields, including biochemistry, molecular biology, medical sciences, plant physiology, agriculture, and related subjects.

• Features step-by-step methods for biochemical analysis of a variety of compounds
• Explores methods that are applicable and adaptable across a variety of fields, including biochemistry, molecular biology, and related areas
• Provides detailed instructions on how to prepare buffers, the equipment to be used, and the analysis of a variety of molecules, including carbohydrates, lipids, proteins, and hormones
• Contains interdisciplinary and adaptable methods and techniques

Inhaltsverzeichnis

• Cover image
• Title page
• Table of Contents
• Copyright
• Preface
• Chapter 1. Introduction to biochemistry
• Chapter 2. Introduction to laboratory procedures
• Chapter 3. Preparation of solutions and buffers
• Chapter 4. Qualitative analysis of carbohydrates
• Chapter 5. Qualitative analysis of proteins
• Chapter 6. pH (negative logarithm of hydrogen ion concentration)
• Chapter 7. Spectrophotometry
• Chapter 8. Estimation of carbohydrates
• Chapter 9. Amino acids and proteins
• Chapter 10. Enzyme assays
• Chapter 11. Vitamin assays
• Chapter 12. Phenolics
• Chapter 13. Plant pigments
• Chapter 14. Plant acids
• Chapter 15. Antinutritional factors in plants
• Chapter 16. Toxins
• Chapter 17. Extraction and identification of polyacetylenes
• Chapter 18. Alkaloids
• Chapter 19. Plant hormones
• Chapter 20. Immunochemical methods
• Chapter 21. Nitrogenase activity
• Chapter 22. Chlorophyll
• Chapter 23. Proximate analysis
• Chapter 24. Minerals
• Chapter 25. Antinutritional factors in plants
• Chapter 26. Antioxidants
• Chapter 27. Lipids
• Index